Proof of transovarial transmission of Lymantria dispar Nucleopolyhedrovirus (fam. Baculoviridae) with the RAPD-PCR method

V. V. Oberemok

Vernadsky Tavrida National University
95007 Simferopol, Vernadsky Avenue, 4, Crimea, Ukraine
e-mail: genepcr@mail.ru

Transovarial transmission of Lymantria dispar Nucleopolyhedrovirus is shown with the RAPD-PCR method. Genetic markers of virus DNA found with the primer OPA-08 are recommended for diagnostics of Lymantria dispar nucleopolyhedrosis, in particular at the egg stage. Genetic markers of virus DNA found with the use of OPA-08 primer are helpful for estimation of genetic diversity of virus, level of the virus presence among the eggs, and for a decision about forestry protective measures.