The congruence between intra-nasal aerodynamics and functional heterogeneity of olfactory epithelium

Volume 78, N 1. 2017 pp. 13–24

A. V. Romashchenko 1,2, D. V. Petrovsky 1, M. P. Moshkin 1,3

1Institute of Cytology and Genetics, Siberian Branch of RAS
630090 Novosibirsk, Academician Lavrentyev, JO
2Design and Engineering Institute of Computer Science, Siberian Branch of RAS
630090 Novosibirsk, Academician Rihanov, 6
3Tomsk State University
634050 Tomsk, Pr. Lenina, 26
e-mail: mmp@bionet.nsc.ru

Zonal organization of the olfactory system depends not only on the pattern of olfactory receptor genes expression but also on the geometry of nasal duct, where receptors to more muco-soluble compounds are concentrated within the dorsal part (the region where airflow velocity is maximal) while receptors to less volatile compounds are located in the ventral part. Increasing of airflow velocity in separate parts of nasal cavity allows, on the one hand, to promote perception of olfactory signals by the receptors while, on the other hand, magnifies the risk of those parts being exposed to different pathogens hold by the airflow, due to their more intensive subsidence. In the present study, we have shown, using manganese-induced magnetic resonance tomography (MRT), that after intra-nasal introduction of colloidal solution of manganese oxide nanoparticles (NOM, Мn3О4 ), in ventral parts of mice olfactory epithelium the capture of insoluble particles turns out to be more intensive than in dorsal ones. Combined introduction of NOM and specific blockers of cell transport and endocytosis indicates that in nasal cavity particles are captured by means of endocytosis and then conveyed to cells of an olfactory bulb through the intracellular transport. At that, in the dorsal part of olfactory epithelium, as opposed to the ventral one, the main contribution to NOM capturing belongs to clathrin-mediated type of endocytosis. Thus, it is established that in mice, the two functional parts of olfactory epithelium differing in intensity of submicron aerosol subsidence demonstrate different intensity of insoluble particles capturing from the nasal cavity, and are characterized by different mechanisms of endocytosis. Hence, structural and functional organization of nasal cavity in mice completely meets the principle of adaptive congruence which confines infectious and toxic impacts of nano-aerosols on cells of olfactory epithelium and brain.

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